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Laboratory Technologist – R&D (Reagent Production)

Entebbe, Uganda * Full-time
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Uganda Virus Research Institute (UVRI), Entebbe, Uganda

Category: Health and Medical Total Positions: Location: Entebbe, Uganda Full-time Salary: Not mentioned Posted 1 year ago
KEY DUTIES AND RESPONSIBILITIES
• 1. Reagent Production & Quality Control a) Express, purify, and characterize recombinant antigens (e.g., spike, nucleoprotein) used in humoral assays. • b) Maintain sterile mammalian tissue culture systems for antigen and pseudovirus production. • c) Perform SDS-PAGE, BCA, and endotoxin quantification to evaluate antigen quality and batch consistency. • d) Support pseudovirus generation, titration, and stability testing under GCLP-compliant workflows. e) Document all production steps, QC metrics, and reagent traceability as per SOPs. • 2. Support for Immunological Assays a) Assist with downstream ELISA, ELISpot, and neutralisation assays, ensuring reagents are appropriately prepared, stored, and qualified. • b) Calibrate and QC plate readers, biosafety cabinets, incubators, and other assay-supporting equipment. • c) Coordinate reagent lot reservation and usage schedules to ensure uninterrupted assay pipelines. 3. Laboratory Systems & Compliance a) Uphold GCLP standards by maintaining clean workspaces, validating equipment, monitoring cold chain storage, and backing up data regularly. • b) Participate in EQA schemes, internal QA reviews, and periodic assay qualifications. • c) Implement and update SOPs relevant to antigen production and assay reagent handling. • 4. Sample Handling & Safety a) Receive and store clinical samples in accordance with ethical protocols. b) Ship biological samples to international labs under IATA-compliant conditions. • c) Conduct safety inductions, ensure use of PPE, manage first aid inventory, and contribute to biosafety audits. • 5. Training & Mentorship • a) Train junior staff, interns, or attachments in relevant techniques, including reagent QC and basic immunoassay support. • b) Supervise and mentor Assistant Technologists, when applicable
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QUALIFICATIONS / SKILLS
• Lab Tech R&D a) A Bachelor’s degree in Medical Laboratory Technology or Biological Sciences (e.g., Biochemistry, Molecular Biology). • b) Minimum one year of hands-on lab experience with antigen expression, purification, or immunological assays. • c) Demonstrated training or experience in GCLP, biosafety, or sample shipping (IATA). d) A credit in Mathematics, Physics, Biology, and Chemistry at O & A Level. e) Strong skills in data entry, record keeping, and Microsoft Office; statistical knowledge is desirable
HOW TO APPLY

Please follow the application instructions provided in the full job description above.

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Deadline: Expired
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